qGW, a Stable and Major QTL for Increasing of Grain Weight in Rice (Oryza sativa L.)

In order to analyze the genetic components of agronomic traits among 116 F9 recombinant lines derived from crosses of Ahlamitarom × Sepidroud rice cultivars, an experiment was conducted as a randomized complete block design in research farm of Gonbad Kavous University of Agriculture with three replications in 2016 and 2017. Genetic linkage map provided with 80 SSR markers, 28 iPBS Markers (79 polymorphic alleles), 7 IRAP markers (17 polymorphic alleles) and 26 ISSR markers (70 polymorphic alleles), which covered 1275.4 cM of the rice genome. QTL analysis was performed by Composite Interval Mapping. In two years, 15 QTLs detected for the studied traits. The additive effected varied from 6.725 g for grain weight up to -85.626 g for grain weight. Also, R2 for the detected QTLs explained from 11.3% to 20% of the total variation. The highest R2 was related to grain weight in the first year of experiment. Among the detected QTLs, qGWs on chromosome 1, were found to be stable and large effector QTLs for rice (Oryza sativa L.) grain weight, and can be used in marker-assisted breeding and selection programs after validation.